Single-molecule analysis of transcription factors and co-factor action in live cells

DIEGO M. PRESMAN

Bethesda, MD

Conferencia; Frontiers in light microscopy; 2017

- SMT confirm that many transcription factors and cofactors are highly dynamic during their chromatin binding activity- Only a small proportion of molecules are specifically/functionally bound at any given time.- Acquisition parameters bias. Photobleaching. Only relative comparisons are valid. We are currently re-evaluating the bi-exponential model- Next steps: 2-color SMT: arrival-departure dynamics of complexes