ANTIOXIDANT AND ANTIPROLIFERATIVE ACTIVITIES OF EXTRACTS FROM THE WASTES OF PECAN NUT WITH POTENTIAL NUTRACEUTIC APPLICATIONS

RIBAS, L. E.; GASSER, F.B.; PERELLO, A.; SIMONETTO, C.; BARAVALLE, M.E.; SAVINO, G.H.; ORTEGA, H.H.; VAN DE VELDE, F.; HEIN, G.J.

Mar Del Plata

Congreso; REUNIÓN ANUAL DE SOCIEDADES DE BIOSCIENCIA; 2023

The waste from the pecan nut industry contains high contents of phenolic compounds with proven biological activities. We aimed to evaluate the in vitro bioactivity of enriched extracts from pecan nut shells and husks on the prostate cancer cell line PC3. Extracts were obtained through an optimized aqueous extraction process and purified by using SPE C18 columns (PES: shell and PEH: husk). The content of total phenolics (TP) was determined by the Folin-Ciocalteu method (mg of gallic acid equivalent/g) and the antioxidant capacity by the DPPH method, determining the relative antioxidant activity (%AAR; IC50 Extract/IC50 Trolox). The cytotoxic activity was studied by evaluating cell viability through the MTT assay with increasing concentrations of extracts (10-300 mg/L) and Doxorubicin (DO) as the positive control, for 48 h. Lactate dehydrogenase (LDH) activity released in the damaged cells was evaluated using a commercial kit (Wiener Lab) in the supernatant of the cell culture incubated with 200 mg/L of each extract, DO, and triton as a positive control. In addition, the %AAR of extracts at 200 mg/L and their combination with DO was evaluated by flow cytometry, analyzing the production of reactive oxygen species (ROS).TP values were 80 mg/g and 3.8 mg/g, and the %AAR were 196.70 and 189.52, for the PES and PEH extracts, respectively. Both extracts demonstrated antioxidant activity (p