RELEVANCE OF SURFACE HYDRATION ON BIOMEMBRANE FUNCTION AS DERIVED FROM MONOLAYER STUDIES

MARTINI, F; HOLLMANN, AXEL; DISALVO, ANIBAL

CORDOBA, ARGENTINA

Congreso; Kinetics and thermodynamics of the adsorption process of aqueous soluble proteins to lipid monolayers; 2009

In this work we have tested the effect of water at different region on the surface pressure of lipid monolayers using two experimental model systems chosen arbitrarily in order to generalize the proposal:  An aqueous soluble protein, Rennet from Mucor miehei, on monolayers composed by zwitterionic phospholipids. In this system, the interface was varied from phosphoethanolamine and phosphocholine and the hydrocarbon core by changing the length, saturation and ramification.  The other system consists of a protein that is normally attached to the surface of cell, the S-layer of Lactobacillus brevis. In this case, the interface composition was SoyPC:Chol:SA, and varied by increasing ratios of stearylamine (SA) on fixed proportions of cholesterol (Chol) and lecithin (SoyPC). In addition, at constant SA, the hydrocarbon core was varies by changing the ratio of Chol to lecithin.