Kluyveromyces marxianus ORAL ADMINISTRATION FOR PREVENTION OF TUMORIGENESIS IN A COLITIS-ASSOCIATED CANCER MURINE MODEL

MALENA MARÍA FERREYRA COMPAGNUCCI; GERÓNIMO DUCCA; JUAN MARCOS MUCCI; FAVIO NICOLÁS CRUCES; MARÍA DOLORES PENDÓN; GRACIELA GARROTE; DAVID ROMANIN; MARTIN RUMBO

Ouro Preto

Congreso; XLVII Annual Meeting of the Brazilian Society of Immunology,in Ouro Preto - MG; 2023

Brazilian Society of Immunology

Colorectal cancer (CRC) is the third most prevalent cancer worldwide. It is known that chronic inflammation increases the risk of tumor development. Our group has demonstrated that Kluyveromyces marxianus CIDCA 8154 strain (Km 8154) has immunomodulatory properties and can control intestinal inflammation in an acute model. Therefore, here we evaluate if its administration impacts the process of tumorigenesis in a murine model of colitis-associated colorectal cancer by administration of azoxymethane/dextran sodium sulfate (AOM/DSS). After intraperitoneal injection of AOM 10 mg/kg, the AOM/DSS group were provided with 2,5% DSS in drinking water for 5 consecutive days, followed by 16 days of regular drinking water. This cycle was repeated 4 times. Mice were administered a yeast suspension on gelatin (1-3 10 UFC/cage) every 48 hours, control mice received gelatin without yeast. Mice were monitored daily and euthanized at week 13. Macroscopic inflammation parameters, such as colon length and liver weight, were measured. No difference in these markers nor in the weight fluctuation between the AOM/DSS groups with or without Km 8154 were found. We counted the presence of polyps in the colon, finding that there are fewer polyps in the group AOM/DSS+Km 8154 compared to the control AOM/DSS group (p4mm whilst none of the AOM/DSS+Km8154 group has more than 4 big polyps. We also administrated heat killed yeast, observing that it has not the same effect of the living form. Our results indicate that dietary administration of Km 8154 could be a positive intervention in prevention of inflammation-induced colorectal cancer, as we found fewer and smaller polyps in the group which has received probiotic yeast.