IMPLICATION OF PROBIOTIC BACTERIA ON ACENOCOUMAROL METABOLIZATION

FRAGOMENO, MELISA; PERUZZO, PABLO J; MINNAARD, JESSICA; PEREZ, PABLO F

Congreso; LVII SAIB Meeting ?XVI SAMIGE Meeting; 2021

Probiotic-based foods are becoming highly popular, and their consumption is growing steadily. On the other hand, very little information is available about the interaction of these products with drugs, including anticoagulants, such as the warfarin derivative acenocoumarol (AC). AC is known to interact with some food components. Previous studies by our group demonstrated that incubation of acenocoumarol with two strains of bifidobacteria (B. bifidum CIDCA 5310 and B. adolescentisCIDCA 5317) reduced the drug concentration after 16 h incubation. In order to deepen our knowledge in this field, different studies were carried out. Moreover, another probiotic bacterium (Lactobacillus acidophillus ATCC 314) was included to examine if the effect was genus dependent. Strains were culture in MRS broth at 37°C for 24 h in anaerobic conditions. Then,strains were washed with PBS and incubated with AC 0.16 mg ml-1 for 1h. A reduction in AC concentration was observed only for the strain CIDCA 5317 (0.14 mg ml-1 ± 8.5 x 10-4). In other experiments, the strains were sonicated for 5 or 8 min and then incubated 1 h with AC 0.16 mg ml-1. The three strains were able to lower the drug concentration and this effect was more evident in samples sonicated for 8 min. The values obtained for strains CIDCA 5310, CIDCA 5317 and ATCC 314 sonicated 8 min were 0.06 mg ml-1 ± 0.015, 0.03 mg ml-1 ± 2.5x10-4 and 0.03 mg ml-1 ± 0.017 respectively. Finally, the three strains were grown at different times (16, 18 and 24h) at 37°C in MRS broth, and then incubated 1 h with AC 0.16 mg ml-1. All incubations were carried out in anaerobic conditions. Here we could see that the three strains were able to lower down the anticoagulant concentration. As an example, for strain CIDCA5310 the values obtained were 0.006 mg ml-1 ± 5.0x10-4 for 16 h-old cultures and 0.004 mg ml-1 ± 3.0x10-3 for 18 h-old cultures. For 24 h-old cultures, the peak of AC in HPLC was present but not quantifiable. These last results were also observed for strains CIDCA 5317 and ATCC 314. Student t-Test was used for statistical analysis. Results showed here suggest that intracellular factors might play a role in the biomodification of the drug and that the physiologic status of bacteria is relevant for the enzymatic activity altering the drug. The effects observed at short periods of time are significant in the context of physiological interaction between microorganisms and xenobiotics in the gastrointestinal tract. In conclusion, the strains under study were able to modify acenocoumarol.