INVESTIGADORES
MOZZI Fernanda Beatriz
artículos
Título:
Hydrolysis of whey proteins by Lactobacillus acidophilus, Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus grown in a chemically defined medium
Autor/es:
M. PESCUMA; E. M. HÉBERT; F. MOZZI; G. FONT DE VALDEZ
Revista:
JOURNAL OF APPLIED MICROBIOLOGY
Editorial:
Blackwell Publishing
Referencias:
Lugar: Oxford, Inglaterra; Año: 2007 vol. 103 p. 1738 - 1746
ISSN:
1364-5072
Resumen:
Aims: To evaluate the ability of themophilic lactic acid bacteria (LAB) to
hydrolyse the whey proteins b-lactoglobulin (BLG) and a-lactalbumin (ALA) in
a chemically defined medium (CDM).To evaluate the ability of themophilic lactic acid bacteria (LAB) to
hydrolyse the whey proteins b-lactoglobulin (BLG) and a-lactalbumin (ALA) in
a chemically defined medium (CDM).b-lactoglobulin (BLG) and a-lactalbumin (ALA) in
a chemically defined medium (CDM).
Methods and Results: The ability of three LAB strains to hydrolyse BLG and
ALA was studied in a CDM supplemented with these proteins or whey protein
concentrate (WPC). Protein hydrolysis was determined by Tricine ⁄ SDSPAGE
and RPHPLC. Maximum BLG (21%) and ALA (26%) degradation by LAB
was observed using WPC. Under starving conditions, BLG degradation was
greater for Lactobacillus delbrueckii ssp. bulgaricus CRL 454 than for Lactobacillus
acidophilus CRL 636 and Streptococcus thermophilus CRL 804. All three
strains showed different peptide profiles and were not able to hydrolyse ALA
under starvation.The ability of three LAB strains to hydrolyse BLG and
ALA was studied in a CDM supplemented with these proteins or whey protein
concentrate (WPC). Protein hydrolysis was determined by Tricine ⁄ SDSPAGE
and RPHPLC. Maximum BLG (21%) and ALA (26%) degradation by LAB
was observed using WPC. Under starving conditions, BLG degradation was
greater for Lactobacillus delbrueckii ssp. bulgaricus CRL 454 than for Lactobacillus
acidophilus CRL 636 and Streptococcus thermophilus CRL 804. All three
strains showed different peptide profiles and were not able to hydrolyse ALA
under starvation.⁄ SDSPAGE
and RPHPLC. Maximum BLG (21%) and ALA (26%) degradation by LAB
was observed using WPC. Under starving conditions, BLG degradation was
greater for Lactobacillus delbrueckii ssp. bulgaricus CRL 454 than for Lactobacillus
acidophilus CRL 636 and Streptococcus thermophilus CRL 804. All three
strains showed different peptide profiles and were not able to hydrolyse ALA
under starvation.Lactobacillus delbrueckii ssp. bulgaricus CRL 454 than for Lactobacillus
acidophilus CRL 636 and Streptococcus thermophilus CRL 804. All three
strains showed different peptide profiles and were not able to hydrolyse ALA
under starvation.CRL 636 and Streptococcus thermophilus CRL 804. All three
strains showed different peptide profiles and were not able to hydrolyse ALA
under starvation.
Conclusions: The assayed LAB strains were able to degrade BLG during growth
in a CDM and under starving conditions. The different peptide profiles
obtained indicate distinct protease specificities.The assayed LAB strains were able to degrade BLG during growth
in a CDM and under starving conditions. The different peptide profiles
obtained indicate distinct protease specificities.
Significance and Impact of the Study: These strains could be used as adjunct
cultures to increase BLG digestibility in whey-based or whey-containing foods.
To our knowledge, this is the first report on the ability of a Lact. acidophilusThese strains could be used as adjunct
cultures to increase BLG digestibility in whey-based or whey-containing foods.
To our knowledge, this is the first report on the ability of a Lact. acidophilusLact. acidophilus
strain to degrade BLG.