Production of endocellulase by Cellulomonas sp. using experimental design

THAIS DEMARCHI MENDES; SOFÍA SAMPAOLESI; T.F. CHAN SALUM; M. C. TRICHES DAMASO

Mar del Plata

Congreso; VIII Encuentro para Latino América y el Caribe de Biotecnología REDBIO; 2013

REDBIO Argentina

PRODUCTION OF ENDOCELLULASE BY Cellulomonas sp. USINGEXPERIMENTAL DESIGNThais Demarchi Mendes1; Sofía Sampaolesi2; Thaís Fabiana Chan Salum1; MônicaCaramez Triches Damaso1; Paola Talia2, Eleonora Campos21 Embrapa Agroenergy, Parque Estação Biológica s/n, Av. w3 Norte (final),CEP 70770-901, Brasília-DF, Brazil. 2 Instituto de Biotecnología, CICVyA, CNIA, INTA Castelar, Argentina.Ethanol is recognized as one of the most important renewable fuels due to theeconomic and environmental benefits of its use. Agro-industrial residues have great potential to be converted in ethanol and this transformation is mediated by enzymatic complex entitled cellulases. Argentine National Institute of Agricultural Technology and Embrapa Agroenergy teams are screening bacteria strains isolated from different biomes with the goal of identifying good cellulase producers. In this work, the effects of process variables on cellulase production by Cellulomonas sp. have been studied using sugar cane bagasse, elephant grass and carboximetilcellulose synthetic substrate aiming to obtain higher levels of cellulases. A fractional factorial design was carried out to test the effect of six variables on the enzymes production: temperature, pH and the concentration of substrate, yeast extract, NaNO3, and inoculum. After 72 and 96 h of fermentation, each sample was taken for enzyme measurement. Exoglucanase and B-glucosidase activities were not detected. Until now, the best results obtained were using sugar cane bagasse after 96 h of fermentation, withendocellulases activities (330 mU/mL) up to nine times higher than those obtained with synthetic substrate.Financial Support: Embrapa, INTA