use of killer biotyping in an ecological survey of yeast in an old patagonian winery

SANGORRIN, M.P.; ZAJONSKOSVKY, I.; CABALLERO, A.C.; GIRAUDO, M.R:

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

SPRINGER

Lugar: Berlin; Año: 2002

0959-3993

An ecological study of Saccharomyces cerevisiae strains in spontaneous alcoholic fermentation has been made in the same winery on two consecutive years (1993 and 1994) with Merlot type musts, and with Malbec type must on a third year (1998). S. cerevisiae strains associated with winery surfaces were also analysed. Differential killer sensitivity patterns related to a killer reference panel of 10 killer yeasts belonging to 9 species of 4 genera were used as a quick and simple procedure to discriminate between S. cerevisiae wild isolates at the strain level. Although a great diversity of wild strains was observed, two main indigenous S. cerevisiae strains, designated as S. cerevisiae 9 and S. cerevisiae 13, took over the Merlot type fermentation in both years. This strains also appeared in Malbec must fermentation during year 1998 and they were again found on the winery surface the next year. This results evidence that over the time period studied (1993-1999) there existed in the winery two indigenous S. cerevisiae which were dominant and stable during vinification and they represent an additional confirmation of the taking over of musts by local strains of S. cerevisiae.