INVESTIGADORES
PONSONE Maria Lorena
artículos
Título:
Statistical optimization of simple culture conditions to produce biomass of an ochratoxigenic mould biocontrol yeast strain
Autor/es:
PELINSKI R.; CERRUTTI P.; PONSONE M.L.; CHULZE, S.N; GALVAGNO M.
Revista:
LETTERS IN APPLIED MICROBIOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2012 vol. 54 p. 377 - 382
ISSN:
0266-8254
Resumen:
Aim: To maximize biomass production of an ochratoxigenic mouldcontrolling
strain of Lachancea thermotolerans employing response surface methodology
(RSM).
Methods and Results: Using PlackettBurman screening designs (PBSD) and
central composite designs (CCD), an optimized culture medium containing
(g l)1): fermentable sugars (FS), 139Æ2, provided by sugar cane molasses
(CMz), (NH4)2HPO4 (DAP), 9Æ0, and yeast extract (YE), 2Æ5, was formulated.
Maximal cell concentration obtained after 24 h at 28C was 24Æ2 g l)1cell dry
weight (CDW). The mathematical model obtained was validated in experiments
performed in shaken-flask cultures and also in aerated bioreactors. Maximum
yield and productivity values achieved were, respectively, of 0Æ23 g
CDW⁄ g FS in a medium containing (g l)1): FS, 87Æ0; DAP, 7Æ0; YE, 1Æ0; and of
0Æ96 g CDW l)1 h)1 in a medium containing (g l)1): FS, 150Æ8 plus DAP, 6Æ9.
Conclusions: Optimized culture conditions for maximizing yeast biomass production
determined in flask cultures were applicable at a larger scale. The highest
yield values were attained in media containing relatively low-CMz
concentrations supplemented with DAP and YE. Yeast extract would not be
necessary if higher productivity is the aim.
Significance and Impact of the Study: Cells of L. thermotolerans produced aerobically
could be sustainably produced in a medium just containing cheap carbon,
nitrogen and phosphorus sources. Response surface methodology allowed
the fine-tuning of cultural conditions.