INVESTIGADORES
PICCOLI patricia Noemi
artículos
Título:
Metabolism of terpenes in the response of grape (Vitis vinifera L.) leaf tissues to UV-B radiation
Autor/es:
GIL M; PONTIN M; BERLI F; BOTTINI R; PICCOLI P
Revista:
PHYTOCHEMISTRY
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Año: 2012 p. 89 - 98
ISSN:
0031-9422
Resumen:
This study investigated the terpene profile analyzed by GC-EIMS of in vitro cultured plants of Vitis vinifera exposed to a ?field-like? dose of UV-B (4.75 kJ m-2 d-1) administered at two different fluence rates (low, 16 h at 8.25 μW cm-2, and high 4 h at 33 μW cm-2). Low UV-B incremented the membrane-related triterpenes sitosterol, stigmasterol and lupeol, more noticeable in young leaves, suggesting elicitation of a mechanism for grapevine acclimation. In contrast, accumulation of compounds with antioxidant properties, the diterpenes α and γ tocopherol and phytol, the sesquiterpene E-nerolidol and the monoterpenes carene, α-pinene and terpinolene revealed maximum accumulation under high UV-B, which was accentuated in mature leaves. Also the levels of the sesquiterpenic stress-related hormone abscisic acid (ABA) increased under high UV-B, although 24 h post irradiation ABA concentrations decreased. Such increments of antioxidant terpenes along with ABA suggest elicitation of mechanisms of defense. The adaptative responses induced by relatively low UV-B irradiation as suggested by synthesis of terpenes related with membrane stability correlated with augments in terpene synthase activity