Metabolic profiling strategy for the accurate determination of phenolic compounds from virgin olive oil by liquid chromatography-mass spectrometry: avoiding the need of applying any extraction protocol

OLMO-GARCÍA, LUCÍA; BAJOUB, AADIL; MONASTERIO, ROMINA P.; FERNÁNDEZ-GUTIÉRREZ, ALBERTO; CARRASCO-PANCORBO, ALEGRÍA

Jaén

Simposio; VIII Simposio Científico-Técnico Expoliva 2017; 2017

VIII Simposio Científico-Técnico Expoliva 2017

A LC-MS method involving direct injection of extra-virgin olive oil (EVOO) -after a simple dilution- for determining its phenolic compounds has been developed. Optimization of the most appropriate solvent for sample dilution, selection of the optimum oil/solvent ratio, and establishment of column cleaning strategy and maximum number of injections were some of the most relevant steps. Then, the analytical parameters of the method were evaluated, establishing LOD (from 3.3 to 31.6 µg/L) and LOQ, precision (RSD values for inter-day repeatability were found between 3.49 and 6.12%), and trueness (within the range 89.9-102.3% for 1.0 mg/L) and checking possible matrix effect (which was no significant). Three kinds of calibration were used: external standard, standard addition and calibration in a phenols-free matrix, which was subsequently applied to quantify the phenolic compounds in 16 EVOOs (from 6 cultivars). A total of 21 compounds were determined without the need of using any extraction protocol. Apart from time saving, the greatest advantages of the implementation of the VOO direct injection into the LC (after a simple dilution) are the prevention of partial and selective recovery of some phenolic compounds after the extraction and their possible partial oxidation, or the creation of artificial isomers during the sample preparation.