Phenolic composition of folk medicinal plants with dermatological relevance by core­shell reversed phase liquid chromatography- multiple wavelength detection

ESPINO, M.; MONASTERIO, R.P.; GOMEZ, ROXANA; SILVA, M.F.

Natal

Simposio; ITP & LACE 2014; 2014

Skin is an important organ that protects us from the environmental conditions. It is exposed every day to a large number of agents such as oxidative stress, UV radiation, skin pathogens, etc. that could be the cause of many skin diseases; hyperpigmentation, inflammation, melasma among others. Plants have been traditionally used in medicine. Indeed, the possibility of using plant extracts for skin treatments is a promising area of research. Botanical reports on Argentinian folk medicinal plants with pharmacological relevance have been highlighted; yet there is still limited scientific data to support claims for their effectiveness against dermatological pathologies. In this work, a simple methodology using ultrasound assisted liquid extraction as clean up for herbal medicines (Origanum vulgare, Thymus vulgaris, Morus alba, Matricaria recutita and Larrea Divaricata) has been applied. The determination of phenolic compounds in the extracts was carried out by core-shell reversed phase liquid chromatography- multiple wavelength detection. Phenolic compounds of pharmaceutical interest (syringic acid; gallic acid; tyrosol; catechin; a-hydroxyphenilacetic; 2,5-dihydroxybenzoic acid; caffeic acid; p-coumaric acid; sinapic acid; ferulic acid; apigenin; quercetin; vanillic acid; rutin and cinnamic acid) were successfully separated with a core shell column, Kinetex C18 (100 x 4.6 mm) 2.6 um from Phenomenex in less than 20 mins at a flow rate of 0.5 mL/min.