Addition of inorganic compounds to increase laccase enzyme activity

MOLINA MELISA ANTONELLA; SGROPPO SONIA C.; MILDE, LAURA B.; ZAPATA PEDRO D; FONSECA MARÍA ISABEL

Rondonia

Simposio; I Simpósio de Microbiologia de Rondônia: Saúde, Ambiente e Inovação; 2021

Introduction: Fungal laccases have shown great potential in industrialand environmental applications. Therefore, further study of the propertiesof the laccase enzyme and its behavior with the addition of differentcompounds is essential to address the potential applications of thisenzyme to specific fields. Objective: Evaluate the ability of differentinorganic compounds to increase the activity of fungal laccases.Materials and methods: To study the effects of different compounds onthe activity of laccase recombianant, we work with a laccase from Phlebiabrevispora heterologously expressed. The effects of Iron chloride, Coppersulphate, Calcium carbonate, Zinc chloride, Potassium chloride andAmmonium sulfate on the activity of laccase were determined. Solutionof 0.5mmol L of each compound were tested and the residual enzymaticactivity were measured and compared with control (without compound).Later, the solution which causes the highest laccase activity, was used inconcentrations from 0,5 mmol L to 3 mmoL to quantify the residualactivity. Results: In the production of recombinant laccases assayed onflask, Iron sulfate 1 mmol L showed the highest enzymatic activity withan activity peak of 613.00 EU (μmoL min mL ) at the fourth day ofincubation. The other compounds assayed showed the following activitypeaks: Iron chloride 373.02 UL , Calcium carbonate 256.3 UL , Zincchloride 47.10 UL , Potassium chloride 87.09 UL , Ammonium sulfate101.03 UL . Conclusion: Based on the results of this work, we concludethat the laccase enzyme supplemented with Iron sulfate offers promisingresults for the application of white-rot fungal resources in environmentalremediation, bakery industry, bioremediation, biodegradation and soforth