BECAS
EBERHARDT Agustina
artículos
Título:
Whey protein hydrolysis with microbial proteases: Determination of kinetic parameters and bioactive properties for different reaction conditions
Autor/es:
EBERHARDT, AGUSTINA; LÓPEZ, EMILSE C; MARINO, FERNANDA; MAMMARELLA, ENRIQUE J; MANZO, RICARDO M; SIHUFE, GUILLERMO A
Revista:
INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2021 vol. 74 p. 489 - 504
ISSN:
1364-727X
Resumen:
Whey protein concentrate was enzymatically hydrolysed with the microbial proteases Alcalase® and Flavourzyme® under different experimental conditions. The effect of the reaction variables on the properties of the hydrolysates was evaluated. The pH had a significant effect both on the degree of hydrolysis and on the kinetic parameters. The initial substrate concentration and the enzyme/substrate (E/S) ratio had an influence both on the degree of hydrolysis and on the hydrophobicity index, but not on the bioactive properties of the hydrolysates. The products of hydrolysis obtained with Alcalase® presented the highest degree of hydrolysis, the lowest hydrophobicity index and the best bioactive properties.
