Immobilization of horseradish peroxidase onto electrospun polyurethane nanofiber matrices

MORALES URREA, DIEGO ALBERTO; CARACCIOLO, PABLO CHRISTIAN; HAURE, PATRICIA MÓNICA; CONTRERAS, EDGARDO MARTÍN

POLYMERS FOR ADVANCED TECHNOLOGIES

JOHN WILEY & SONS LTD

Año: 2021

1042-7147

In this work, horseradish peroxidase (HRP) was immobilized onto polyurethanenanofiber membranes. The following variables were optimized to maximize the surfacedensity of grafted HRP (Q): NaClO functionalization time (tf), immobilizationtime (ti), HRP concentration of the immobilization solution (CHRP), and immobilizationtemperature (T). Catalytic activity was evaluated using the decolorization reaction ofOrange II (OII). A statistical analysis of SEM images demonstrates that fiber diameters(d) of native (M), AGE-modified (MA), and HRP immobilized (MAH) electrospun membranes obeyed a log-normal distribution and that the effects of the NaClO activationprocedure, and the use of MAH membranes in OII oxidation during 4 h on the fibersize were negligible. Although obtained membranes at 40C (MAH9540) presentedthe highest Q, matrices obtained at 20C (MAH9520) exhibited the highest catalyticactivity, indicating that HRP was partially inactivated during the immobilization at40C. Reusability tests demonstrated that membranes retained between 11% and33% of their initial enzyme activity after a total reaction time between 4 and 8 h.