PERSONAL DE APOYO
FORNAGUERA Maria Jose
artículos
Título:
Lactobacillus reuteri CRL 1101 highly produces mannitol
Autor/es:
M. E. ORTÍZ; M. J. FORNAGUERA ; R. R. RAYA ; F. MOZZI
Revista:
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2012 vol. 95 p. 991 - 999
ISSN:
0175-7598
Resumen:
Abstract Mannitol is a natural polyol extensively used in
the food industry as low-calorie sugar being applicable for
diabetic food products. We aimed to evaluate mannitol
production by Lactobacillus reuteri CRL 1101 using sugarcane
molasses as low-cost energy source. Mannitol formation
was studied in free-pH batch cultures using 3?10%
(w/v) molasses concentrations at 37 °C and 30 °C under static
and agitated conditions during 48 h. L. reuteri CRL 1101 grew
well in all assayed media and heterofermentatively converted
glucose into lactic and acetic acids and ethanol. Fructose was
used as an alternative electron acceptor and reduced it to
mannitol in all media assayed. Maximum mannitol concentrations
of 177.7±26.6 and 184.5±22.5 mM were found
using 7.5% and 10% molasses, respectively, at 37 °C
after 24-h incubation. Increasing the molasses concentration
from 7.5% up to 10% (w/v) and the fermentation period up to
48 h did not significantly improve mannitol production.
In agitated cultures, high mannitol values (144.8±
39.7 mM) were attained at 8 h of fermentation as
compared to static ones (5.6±2.9 mM), the highest
mannitol concentration value (211.3±15.5 mM) being found
after 24 h. Mannitol 2-dehydrogenase (MDH) activity was
measured during growth in all fermentations assayed; the
highest MDH values were obtained during the log growth
phase, and no correlation between MDH activities and mannitol
productionwas observed in the fermentations performed.
L. reuteri CRL 1101 successfully produced mannitol from
sugarcane molasses being a promising candidate for microbial
mannitol synthesis using low-cost substrate.