INVESTIGADORES
RAYA Raul Ricardo
congresos y reuniones científicas
Título:
Characterization of Lactobacillus curvatus G, a lactocin 705- and antilisteria-deficient mutant
Autor/es:
M.J. OLAYA PASSARELL, C. BELFIORE, R. R. RAYA
Lugar:
San Miguel de Tucumán, Tucumán, Argentina
Reunión:
Simposio; III International Symposium on Lactic Acid Bacteria II Argentinean LAB Net Meeting; 2009
Institución organizadora:
CERELA
Resumen:
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D 14
CHARACTERIZATION OF Lactobacillus curvatus G, A LACTOCIN 705- AND ANTILISTERIA-
DEFICIENT MUTANT
M.J. Olaya
Passarell, C. Belfiore, R. R. Raya
Centro de
Referencia para Lactobacillus (CERELA). Chacabuco 145. 4000 San Miguel de Tucuman,
Argentina. E-mail: mjolaya@cerela.org.ar
Lactobacillus
curvatus CRL705 produces two bacteriocins, Lac705 and AL705, and harbors two
plasmids, pRC18 and pRC12. The gene determinants of lactocin Lac705, genes a705/b705,
imm705, abc705 and acc705, are encoded in pRC18, a plasmid of 18600 bp whose
DNA sequence has been determined. In this study, the characterization of a Lac705-
and AL705- deficient mutant, named strain G, is described. Strain G has been isolated
after transforming L. curvatus CRL705 with plasmid pTV01::Tn917 and growing the
transformants at high temperature (39°C).
Confirmation that strain G derived from CRL705 was determined by API50 analysis
and by RAPD reactions, using primers RAPD1, RAPD2, REP1RI, REP2I and REPV.
Agarose gel electrophoresis showed that plasmids pRC12 and pRC18 were absent in
strain G. However, the presence pRC18 in strain G, and therefore of the
lactocin Lac705 operon, was confirmed by PCR reactions using a set of three
pRC18-specific primers (primers #1/#2, #3/#4 and #5/#6). The abundance and size
of the amplified PCR products suggested that plasmid pRC18 is present in strain
G in a low copy number and that the structural genes of lactocin Lac705 (a705/b705)
are rearranged, which could explain the phenotype observed.
