Lactic acid bacteria isolated from poultry protect intestinal epithelial cells of chicks from in vitro wheat germ agglutinininduced cytotoxicity

BABOT J.D.; ARGAÑARAZ MARTINEZ E.; LORENZO PISARELLO M.J.; APELLA M.C.; PEREZ CHAIA A.

BRITISH POULTRY SCIENCE

TAYLOR & FRANCIS LTD

Lugar: Londres; Año: 2017

0007-1668

1. Whole grain or milled wheat is often used instead 24 of corn in poultry feeding, due to its high content of starch and proteins. Poultry fed with wheat-based diets regularly ingest wheat germ agglutinin (WGA).2. WGA showed toxic effect in vitro on intestinal epithelial cells (IEC) obtained from fourteen-day-old broilers. Cytotoxicity was dependent on time and lectin concentration; lethal dose (LD50) was 8.36 μg/mL for IEC exposed for 2 h to WGA.3. Complementary sugars to WGA were detected on the surface of one Enterococcus and nine Lactobacillus strains isolated from poultry. These strains were evaluated as a lectin removal tool intended to cytotoxicity prevention.4. The incubation of the lactic acid bacteria with WGA before the IEC-lectin interaction caused a remarkable reduction in the percentage of cell death. The protection was attributed to the amount of lectin bound to the bacterial surfaces and was strain dependent. L. salivarius LET 201 and L. reuteri LET 210 were significantly more efficient than the other lactic acid bacteria assayed. 5. These results provide bases for the development of probiotic supplements or cell-wall preparations of selected lactic acid bacteria intended to avoid harmful effects of a natural constituent of the grain in wheat-based diets.