PROBIOTIC BACTERIA CELL WALL AS ORAL ADJUVANTS ON THE MUCOSAL IMMUNE SYSTEM

LEMME DUMIT, JOSÉ MARÍA; PERDIGÓN, GABRIELA.; MALDONADO GALDEANO, CAROLINA

Buenos Aires

Congreso; IV LASID Meeting LXIII Argentinian Society Immunology meeting II French Argentinean Immunology meeeting; 2015

SAI

Receptors presents in gut cells are able to interact with microorganism?s cell wall (CW), molecules that are keys ligands, and induce signaling pathways, resulting in many effects. The aim of this study was to determine the ability to activate mucosal immune system by oral administration of cell wall isolated from probiotic microorganisms. CW fraction from Lactobacillus casei CRL431, Lactobacillus paracasei CNCM I-1518 and Lactobacillus acidophilus CRL730 were obtained. BALB/c mice (5 weeks old) received CW fraction by gavage. Ex vivo, microbicidal activity from peritoneal, spleen and Peyer patches macrophages and cytokines levels (IL-6, IL-10 and MCP-1) from supernatant cultures of intestinal epithelial cells (IECs), were measured. IECs activation was study trough transmission-electron-microscopy (TEM) and the number of goblets cells was determined on histological slices by optical microscope. Microbicidal activity of macrophages was enhanced after CW treatment. Cytokines from IECs showed higher levels of IL-10 and IL-6 after CW administration. Similar results were obtained for the chemokine MCP-1. The micrographs showed an important activation of IECs and goblet cells 30 min after gavage with CW fractions. However there was no increase in the number of goblets cells. IECs are in direct contact with luminal bacteria and immune cells. They can receive and integrate signals. One of the major mechanisms by which probiotics can positively affect the host health, involves the ability to interact with the host immune system. Probiotic components are able to act as oral adjuvant is an approach to elucidate the molecules involved in such effect