Design an economic culture medium from industrial by-products for the development of probiotic poultry strains

BERTANI MS; GRANDE SMM; GARRO M S; PEREZ CHAIA A

Tucuman

Simposio; SIBAL2016 V International Symposium on Lactic Acid Bacteria; 2016

CERELA-CONICET

Probiotic strains are frequently used for poultryfeed, especially lactobacilli and enterococci selected for their potentialbeneficial properties in vivo.  It is currently known that the type of nutrient and selective agents usedin isolation media determine the development of strains, which have characteristichydrophobicity patterns for each medium. Thus, it is suggested that such componentsmay influence the expression of cell-wall glycoproteins and carbohydrates. Theaim of this work was to study the effect of different culture media compositionon growth rate, biomass production and of cell-surface properties of Lactobacillus and Enterococcus strains isolated from poultry intestine. Conventionalculture media (LAPTg, MRS, CAm, PAP) were used as wellas media specifically designed with by-product from local industries such as creamyeast (Cervecería y Maltería Quilmes), cane molasses (Estación ExperimentalAgroindustrial Obispo Colombres EEAOC), milk whey and cheese whey (Trancas,Tucumán). Three strains were tested: E.faecium LET 301, L. salivariusLET 201 and L. reuteri LET 210. Theywere inoculated in all media and incubated at 37°C. In all cases, biomass wasdetermined by viable cell counts in agar media. Growth rate and cell-surfaceproperties were also assessed. Hydrophobicity was evaluated by partition assaywith three different solvents (toluene, p-xylene and n-hexadecane). Autoaggregationtest was performed by measuring variations of OD600 nm of cellularsuspensions every 1 h during 4 h. All strains showed similar or higher growthin alternative medium compared to control media (LAPTg for Enterococcus and MRS for lactobacilli). E. faecium LET 301 showed similar hydrophobicity in all mediaevaluated, showing a low hydrophobicity or non-hydrophobic character, whateverthe solvent used. The hydrophobic character of L. salivarius LET 201 was medium and high when assayed towardp-xylene, and medium and low toward toluene and n-hexadecane, respectively. L. reuteri LET 210 presented lowhydrophobicity in all solvents assay. None of the strains evaluated evidencedautoaggregation. These results demonstrate that the evaluatedby-products from local industries can be successfully used as substrates in economicculture media for the development of poultry probiotics. Nevertheless, it mustbe taken into account that each strain has different nutritional requirements.