HYPOTHALAMIC MASH1 IS MODULATED BY HIGH-FAT DIET CONSUMPTION DURING PREGNANCY AND LACTATION IN MICE

LEMES, SIMONE FERREIRA; BALIANI, TANYARA DA SILVA; VERSUTTI, MILENA DIORIO; MILANSKI, MARCIANE; TORSONI, ADRIANA SOUZA; TORSONI, MARCIO ALBERTO

Foz do Iguaçu, Paraná

Congreso; 23rd Congress of the International Union for Biochemistry and Molecular Biology (IUBMB); 2015

23rd Congress of the International Union for Biochemistry and Molecular Biology (IUBMB)

INTRODUCTION Studies show that inflammation triggered by high-fat diet-induced (HFD) obesity seems to impair hypothalamic neurogenesis Mash1-mediated. In addition, maternal consumption of HFD during the pregnancy cause metabolic disturbs in adult offspring. OBJECTIVES To evaluate the effects of the maternal HFD consumption on Mash1 in the hypothalamus of mice offspring. MATERIALS AND METHODS Females mice (Swiss) were randomly divided into two groups: one was fed with HFD and other, with standard chow (SC), during pregnancy and lactation. After delivery the litter size (SC offspring, SC-O; HFD offspring, HFD-O) was reduced to 8 animals per dams. Pups (28 days old-P28) were euthanized and the hypothalamus was collected to evaluate gene expression of MASH1 (Ascl1) and Hes5 by qPCR. The hypothalamic arcuate nucleus of P28 mice was collected by punch to evaluate the same genes. Data are expressed as means ± SEM, the groups were compared with the Student?s t-test. Statistical significance for all analysis was set at p