IMMUNOMODULATOTY EFFECTS OF EXTRACTS OF ORIGANUM VULGARE (OREGANO) AGAINT HELICOBACTER PYLORI

SALINAS IBÁÑEZ AG, ; CANALE F, ; ARISMENDI SOSA AC,; MATTAR MA, ; ALARCÓN T, ; VEGA AE

Barcelona

Congreso; European Society of Clinical Microbiology and Infectious disseases Congress; 2014

Sociedad Europea de Microbiologia Clinica y Enfermedades Infecciosas

Objetive: Nitric oxide (NO) production can kill Helicobacter pylori, but induction by the microorganism of MO arginase II inhibits inducible NO synthase (iNOS) translation and restricts bacterial killing. Arginases are enzymes, natural competitive inhibitors of iNOS, because they metabolize the same substrate, L-arginine. Taking this into account, the aim was to evaluate in vivo immunomodulatory effect of oregano extract (OE) in both infected and uninfected MO with H. pylori.Methods: Groups of four BALB/c mice were injected intragastrically twice in a 48 h period with 250 µl OE (1mg/ml). As control group, animals received 250 µl of PBS. Peritoneal cells were harvested from each mouse. Morphology of MO was evaluated by Giemsa staining. The production of reactive oxygen species (ROS) was evaluated with the nitroblue tetrazolium (NBT) assay and we used 1 mg/ml opsonized zymosan (OPZ) to stimulate the oxidative burst. Arginase activity and NO was determined in peritoneal MO uninfected and infected with H. pylori (ratio 1 MO: 10 bacteria). Results: OE did not induce apoptosis in MO. Compared with the control infected group, the infected treated with OE mice showed a higher NBT reduction value (p