DIAGNOSIS OF Helicobacter pylori IN SALIVA. DETECTION OF CLARITHROMYCIN RESISTANCE

SALINAS, AG;; CORTIÑAS TI; ; LUCERO ESTRADA C;; VEGA AE

San Juan

Congreso; 2da Reunión Conjunta de Sociedades de Biología; 2011

Sociedad de Biología de Cuyo

Helicobacter pylori colonize the gastric mucosa and leads to the development of chronic gastritis, peptic ulcer and gastric cancer. The presence in the oral cavity is considered as a source of infection and re-infection after antimicrobial therapy, as well as intrafamily transmission. The aim of the study was to determine the sensitivity of PCR for diagnosis of H. pylori from saliva samples and detection of clarithromycin resistance (CLA-R) genotypes. PCR sensitivity was determined using serial dilutions of a saliva sample from H. pylori negative patient artificially contaminated with 1.5x106 ufc/ml of NCTC 11638 H. pylori reference strain. For diagnosis of infection and detection of CLA-R genotypes, 2-3 mL of saliva samples from each of four family members: two parents symptomatic and diagnosed H. pylori positive and two children non symptomatic. The specie-specific antigen was used for diagnosis of infection. Genotypes of CLA-R were determined by RFLP-PCR method using BsaI and MboII enzymes. Limit detection of H. pylori from saliva sample was 102 ufc/ml. The detection in saliva confirmed the presence of bacteria into oral cavity of parents, and one of the boys. Mother and son showed A2143G genotype, while father was CLA-S. The results showed that the saliva may serve as an effective and valuable noninvasive sample to diagnose of infection and monitor the CLA resistant strains.