Detecting of Helicobacter pylori by Polymerase Chain Reaction (PCR) in water artificially contamined

SALINAS IBAÑEZ AG, ; JAURE O, ; SILVA HJ, ; VEGA AE

Mendoza

Congreso; Reunión Científica Conjunta de la Sociedad de Biología de Cuyo; 2010

Sociedad de Biología de Cuyo

Helicobacter pylori is a gram-negative microaerophilic bacterium that infect human gastric epithelial cell surfaces and the overlying gastric mucin, which is a highly specialized niche. It is the major cause of peptic ulcers and a contributor to other illnesses, such as gastric cancer. Also is known to be an agent causing susceptibility to other food and waterborne pathogens. The mode of transmission of H. pylori is still unresolved; some epidemiological data suggest water as a possible transmission route. The aim of the study was to validate the sensitive of PCR for rapid detection of H. pylori from environmental samples. The reference strain of H. pylori (NCTC 11638) was used in this study. Samples of 100 ml of sterile river water were artificially contaminated with a suspension of H. pylori (1.5 x 108 ufc/ml) and the sensitivity of PCR was assayed with serial dilution. DNA extraction was performed with three different methods to test the most effective. Three set of primer that codified by ureA, flaA and 16SRNA was assayed. The result obtained showed that the PCR method allowed detecting H. pylori up to 10- 4 dilution and all primer allowed the detection. The water has a key role in the transmission of H. pylori infection and his presence should be controlled in the aquatic environments to prevent the spread of the infection.