Detection HLA-DQ alleles in patients with Celiac Disease and Helicobacter pylori infection.

DENITA JUÁREZ SP, ; SALINAS IBÁÑEZ AG, ; ARISMENDI SOSA AC,.; VEGA AE

Mendoza-Maipu

Congreso; XXXI Reunión Anual de la Sociedad de Biología de Cuyo; 2013

Sociedad de Biología de Cuyo

Celiac disease (CD) is an autoimmune disorder associated with a small bowel lesion induced by toxic gliadin components and is strongly associated with human leukocyte antigen (HLA)-DQ2 or DQ8. Helicobacter pylori is a bacteria inhabit gastric mucosa and it can lead chronic inflammation, peptic ulcers and gastric cancer. The aim of this study was to determinate the association between alleles DQA10501 and DQB10201 in the molecular diagnosis of CD and its association with H. pylori infection. A total of 27 patients were included in the study, which 11 have CD diagnosis based on histological study and endomysial or transglutaminase antibodies. For the extraction of genomic DNA standard proteinase K and phenol-chloroform method was used. PCR using specific primers for diagnosis of DQ2 alleles and culture, histology and urease test for the detection of H. pylori was performed. The HLADQ2 allele was identified both in 91% of patients with celiac disease diagnosis and six no CD (22%). The results showed 80% of prevalence of H. pylori infection in celiac patients with chronic antral gastritis and villous/cript VC ratio grade 3 and 4. In addition four patients without CD presented H. pylori infection. Molecular HLA-DQ detection is relevant because CD diagnosis based on histology and disease-specific antibodies is not always reliable. Patients that carry HLA-DQ2 could suffer CD at some stage in their lives. Moreover in patients colonized with H. pylori for which the infection could determine the evolution of CD.