ANTI- Helicobacter pylori ACTIVITIES OF Origanum vulgare AND Larrea divaricata CAV EXTRACTS IN VITRO

FERRAMOLA FF, ; SALINAS IBAÑEZ AG, ; ARISMENDI SOSA AC, ; MATTAR DOMINGUEZ MA,.; VEGA AE.

Buenos Aires

Congreso; Reunión conjunta de Sociedades de Biociencias; 2017

Sociedades de Biociencias

Helicobacter pylori is a gram-negative bacillus and is the causeof peptic ulcer disease and gastric cancer. Due to antibiotic resistance,there is great interest in the search for new therapies. Ureaseand Flagella are virulent factors that are required for the colonizationand survival of H. pylori in the human stomach. Origanum vulgare(Orégano) and Larrea divaricata Cav. (Jarilla) are plants known fortheir properties in folklore medicine. The aim of this work was toinvestigate the effects of O. vulgare and L. divaricata extracts onantimicrobial activity and inhibition of urease and flagella activities inH. pylori strain. In this study, the antibacterial activity of both aqueousextract against H. pylori strain was assayed according to CLSIguidelines (CLSI, 2007). The extracts were diluted to obtain the finalconcentrations from 10 to 0,009 mg/ml for each. Serial dilutions ofAmx (Sigma-AldrichCo., StLouis, MO) were used as a control in thesusceptibility test. Bacterial suspension was adjusted to a scale of0.5 McFarland standard (1x108 CFU/ml). Minimal inhibitory concentration(MIC) was measured by determining the lowest amount ofextract or antibiotic needed to inhibit the visible growth of the microorganism.The reference strain HP146128 was treated with sub-inhibitoryconcentrations of Orégano extract (0,5 mg/ml) and Jarillaextract (1 mg/ml) and was incubated for 26h at 37°C in microaerophilicatmosphere. The gene expressions were determined by RTPCRusing 16s RNA as housekeeping gene. The results obtainedshowed that the MIC of Orégano and Jarilla were 1,25 mg/ml and2,5 mg/ml respectively. Orégano extract down-regulates the ureAgene and fla gene expressions; while the Jarilla extract showed nodecrease in the expression of the same genes to the concentrationassayed. The use of Orégano extract can contribute as alternative and complementary medicines, safe and less costly in the treatmentof H. pylori infections.Keywords: Helicobacter pylori, Origanum vulgare, Larrea divaricataCAV, Antimicrobial activity.