BECAS
SALINAS IBAÑEZ Angel Gabriel
congresos y reuniones científicas
Título:
SEROTYPES, ANTIMICROBIAL SUSCEPTIBILITY AND CLONAL RELATEDNESSS OF SHIGA TOXIN-PRODUCING Escherichia coli (STEC) STRAINS RECOVERED FROM BOVINE SAUSAGES
Autor/es:
SALINAS IBÁÑEZ AG, ; FAVIER GI, ; LUCERO ESTRADA C, ; MATTAR MA, ; STAGNITTA P, ; VEGA AE, ; MILIWEBSKY E,; ZOLEZZI G, ; CARBONARI C, ; ESCUDERO ME
Lugar:
Mendoza
Reunión:
Congreso; XXXIV Reunión Científica Anual Sociedad Biología de Cuyo.; 2016
Institución organizadora:
Sociedad de Biología de Cuyo
Resumen:
Shiga toxin-producing Escherichia coli (STEC) can produce hemorrhagic colitis and hemolytic uremic syndrome (HUS) mainly after consumption of contaminated food of bovine origin. The O157:H7 serotype is frequently implicated in clinical manifestations, and some non-O157 serotypes can also be associated to human infections. Even though the antimicrobial therapy is not recommended, the study of antimicrobial susceptibility of STEC strains has epidemiological relevance. In this work, we studied serotypes, antimicrobial susceptibility and clonal relatedness of one strain (STEC 1) isolated from 52 cooked sausages (one positive sample, 1.9%) and three strains (STEC 2, 3 and 4) isolated from 58 fresh sausages (one positive sample, 1.7%) in San Luis, Argentina. Samples were purchased in different retail stores from August 2012 to July 2013, and subjected to molecular methods and culture for STEC detection. PCR for rfbEO157 and fliCh7 genes and serological reactions to assess serotypes were performed. Antimicrobial susceptibility was assayed by Kirby-Bauer method, and the clonal relatedness between strains was determined by XbaI-PFGE. STEC 1 was serotyped as ONT:H7 (NT: not typeable), STEC 2 was ONT:HNT, whereas STEC 3 and STEC 4 were O112:H19. All the strains showed resistance only to erythromycin. A 72.10% similarity was demonstrated by XbaI-PFGE, and the two STEC O112:H19 strains showed an identical DNA profile. At the present, the O112 serotype has not been involved in STEC outbreaks or sporadic cases in humans in our region. However, STEC O112 has been previously identified in cattle from Argentina and the presence of this pathogen in
