HIGH SALT CONCENTRATIONS INDUCES A DELAYED ACTIVATION PATTERN IN HUMAN NEUTROPHILS

MAZZITELLI, I.; BLEICHMAR, LUCÍA; MELUCCI GANZARAIN, CLAUDIA; ADAMCZYK, ALAN; A. LOPEZ MALIZIA; A.PALETTA; GEFFNER, J.

Virtual

Congreso; Reunion anual de Sociedades de Biociencia; 2020

SAI SAIC SAFIS

Elevated concentration of salt (sodium chloride) are present in anumber of tissues under physiological and pathological conditions.Previous studies have analyzed the effect of high salt concentrationson the phenotype and function of macrophages and T cells. Here,we analyzed the effects induced by high salt on neutrophil function.Neutrophils were isolated from human blood by conventional procedures. We found that exposure of neutrophils to hypernatremicmedia (400 mOsm) for 30 min induced an early state of neutrophilparalysis characterized by a profound inhibition in the ability of conventional agonists to induce an increased expression of CD11b, calcium transients, the production of IL-8 and the activation of the respiratory burst: % inhibition > 45% for each analyzed response (n = 9,p