Exploring the Colonic Metabolism of Grape and Strawberry Anthocyanins and Their in Vitro Apoptotic Effects in HT-29 Colon Cancer Cells

LÓPEZ DE LAS HAZAS, MARÍA-CARMEN; MOSELE, JUANA I.; MACIÀ, ALBA; LUDWIG, IZIAR A.; MOTILVA, MARÍA-JOSÉ

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

AMER CHEMICAL SOC

Año: 2016

0021-8561

Beneficial properties attributed to the intake of fruit and red wine have been associated with the presence of significant amounts of anthocyanins. However, their low absorption and consequent accumulation in the gut have generated the suspicion that colonic metabolites of anthocyanins are probably involved in their protective effects. Grape pomace and strawberries extracts, rich in malvidin- and pelargonidin-glucoside, respectively, were fermented in-vitro using human feces as microbial inoculum. After 8 h of anaerobic incubation, the anthocyanins were almost completely degraded while their microbial metabolites concentration was highest at 24 h. Syringic acid and tyrosol were the main metabolites of grape and strawberry extracts respectively. Based on the metabolites detected, metabolic pathways of malvidin and pelargonidin glucosides were proposed. Anthocyanin-rich grape and strawberry extracts and their generated metabolites such as hydroxyphenylacetic acid showed apopototic effects in HT-29 colon cancer cells and may suggest their possible contribution as anti-carcinogenic agents.