Sunflower seed-based medium for growth of Ganoderma spp.

CURVETTO N.; R. GONZÁLEZ MATUTE; D. FIGLAS; S. DELMASTRO

Cuernavaca, Morelos, Mexico

Conferencia; Mushroom Biology and Mushroom Products Fourth International Conference; 2002

Ganoderma lucidum and Ganoderma oregonense produce medicinal giant polysaccharides. We attempted to improve liquid culture to produce these polysaccharides in higher amounts. We added 65 g.l-1 sunflower seeds or sunflower seed broth (equivalent to 16.3 g.l-1 seeds) to basal media (malt extract, yeast extract, glucose: 20, 2 and 10 g.l-1, respectively), pH 6.0. G. lucidum was incubated at 25°C and G. oregonense at 22°C, in darkness and shaken at 90 rpm. G. lucidum growth rate was maximal at day 4 and produced higher mycelium concentrations (9.0 mg.ml-1) in both amended media at day 6, with the broth producing the maximum polysaccharide concentration (3.6 mg.ml-1) at day 5. G. oregonense reached maximum growth rate by day 12, with a maximum of 10.3 mg.ml-1 mycelium concentration in the broth medium; also polysaccharide production was much higher (3.4 mg.ml-1 ) in this medium. Both amended media markedly improved the mycelial growth and polysaccharide production rates in G. lucidum while only the broth medium produced a marked increase in both parameters in G. oregonense.