Efficacy and toxicology of silver nanoparticles as a component wound of healing dressing in zebrafish larvae and embryo

SOSA AYELEN; IGARTÚA DANIELA EDITH; GARCIA LORENA; OCAMPO ALEJANDRO; ALONSO SILVIA DEL VALLE; PRIETO MARÍA JIMENA; MARTINEZ CAROLINA SOLEDAD

Congreso; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2020: SAIC-SAI-SAFIS; 2020

Silver nanoparticles (AgNPs) have an important role in nanotechnology since they possess exceptional bactericidal activity. Increasing applications are the AgNP-based wound dressing, providing a large surface area with high bactericidal efficacy. We aimed to design a film of hydrogel, composed of autochthonous and low cost polymers. Within the film, oil-in-water emulsions will be used to encapsulate and delivery AgNPs, vitamins, anesthetic, and anti-inflammatories. This novel device for the treatment of wounds and burns will be enriched with supplements focused in the reduction of pain and in the acceleration of healing process. As a first step, we will carry out an analysis of efficacy of the AgNPs in opportunistic bacterias commonly found on the skin, and toxicology of the AgNPs in zebrafish embryo and larvae. Results will help to select a concentration to next use in the emulsion. To evaluate effectiveness of AgNPs, Staphylococcus aureus and Pseudomonas aeruginosa were incubated with 1.6-60 ppm AgNPs, and minimal inhibitory concentration (MIC) was determined. To evaluate toxicology of AgNPs, Zebrafish embryo and larvae were incubated with 0.1-100 ppm AgNPs, then studies of LC50, acute toxicity (mortality), teratogenicity (hatching rate and morphology), general developmental abnormalities and specific tissue toxicity (brain and heart function) were conducted. The bacterial inhibition indicated a high susceptibility of Staphylococcus aureus and Pseudomonas aeruginosa from 30 ppm. It was observed that AgNPs, in the highest concentrations, caused a delay in the development of embryos, morphological abnormalities in the larvae, decreased swimming and heart rate. In the larval lethality study, it was observed that the LC50 at 1 hs post-incubation was 683 ppm, and at 48 hs post-incubation, it was 108 ppm. The comparison of in vitro and in vivo results allows us to find a functional and harmless AgNPs dose.