Nanotoxicology characterization of carbamazepine-dendrimers complexes.

IGARTÚA DANIELA EDITH; MARTINEZ CAROLINA SOLEDAD; ALONSO SILVIA DEL VALLE; PRIETO MARIA JIMENA

Porto Alegre

Simposio; IV Latin American Zebrafish Network (LAZEN) Symposium; 2016

Introduction: Carbamazepine (CBZ) is on the market as an antiepileptic. It also has potential uses for the treatment of numerous neurodegenerative diseases. However, it has low solubility in aqueous media, inefficient pharmacokinetic profiles and multiple side effects, so it is important to avoid widespread distribution. Drugs delivery systems (DDS) have gained relevance in the field of nano and bio-medicines. PAMAM dendrimers G4.0 and G4.5 are optimal polymers as DDS that would increase the solubility of CBZ and the arrival to the brain.Objectives: Our objective was to characterize complexes between CBZ and dendrimers and to study their nanotoxicity for both human health and environmental risk during production.Methods: In vitro toxicity in human red blood cells was analyzed by hemolysis and in cell culture by colorimetric methods (MTT, crystal violet and neutral red). In vivo toxicity was studied in zebrafish animal model.Results: No hemolytic activity or morphological changes for any of the formulations were observed. As regards in vitro toxicity, different effects were observed for free drug, dendrimers and complexes, which allowed us to predict toxicity mechanism. In vivo toxicity showed no marked toxic effects between free drug and the complexes.Conclusions: It has been developed a DDS based on dendrimers capable of efficiently transport the antiepileptic drug CBZ; what´s more, it is not toxic in in vitro and in vivo models.