Dendrimers Efficency as a Drug Delivery System for Antiepileptic Drug: A biophysical study

IGARTÚA DANIELA EDITH; MARTINEZ CAROLINA SOLEDAD; ALONSO SILVIA DEL VALLE; PRIETO MARIA JIMENA

Edimburgo, Escocia

Congreso; 19th International Union of Pure and Applied Biophysics (IUPAB) and 11th European Biophysical Societies' Association (EBSA); 2017

The use of dendrimers (D) as nanotechnology tools in medicine has soared up in recent years. The PANAM D 4 and 4.5 are specially known as efficient drug delivery systems. In this work the use of these D are studied to enhance the solubility and delivery of the antiepileptic drug Carbamazepine (CBZ). The biophysical characterization included time stability, endurance to a lyophilization process, D-CBZ interaction by FTIR, and kinetic release profile of the drug by microdialysis. The complexes obtained resulted in drug delivery systems were the ratio CBZ: D was 20:1, which presented stability against the lyophilization process, had a controlled drug release, where interaction was mainly based on hydrophobic forces. In addition, the complexes were tested in vitro (cell lines Neuro2A), ex vivo (human blood cells) and in vivo (larvae Zebrafish D. rerio). No hemolytic effect was observed in the ex vivo model. Contrary to this, there was a toxic of the CBZ in both in vitro and in vivo studies, but not of the D-CBZ complex: the toxic action of the drug was predicted. The D as a nanovehicle in this study resulted in an efficient nanovehicle to bind CBZ. The potentiality relays on their ability to significantly prevent the toxic effects of the drug in both in vitro and in vivo models.