Polyphenolic profiles of cider and dessert apple varieties achieved in Spanish breeding programs: Influence of the genitors and the harvest year on the progeny

SASÍA-ARRIBA, ANDREA; ALONSO SALCES, ROSA MARIA; DAPENA, ENRIQUE; BERRUETA, LUIS A.; GALLO, BLANCA

Madison

Conferencia; XXIXth International Conference on Polyphenols; 2018

Groupe Polyphénols

The main objective of the breeding programs led by the Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA) in Villaviciosa (Asturias, Spain) and the Fruitcentre from Investigación y Tecnología Agroalimentarias (IRTA) in Lleida (Cataluña, Spain), which aims the development of new cider and dessert apple varieties, respectively, of high interest in terms of fruit quality, resistance to scab, powdery mildew, rosy apple aphid, low susceptibility to fire blight and regular bearing, so that they could adapt to the environmental conditions of Mediterranean (Ebro Valley), Atlantic (Cantabrian coast) and/or mountainous regions.In the present study, cider apples were harvested at the maturity stage along three harvest seasons at the SERIDA, and dessert apples along two harvest seasons at the IRTA.The detailed phenolic composition of the apple juices studied were determined by ultrahigh performance liquid chromatography (UHPLC) coupled to a diode array detector (DAD), electrospray ionization (ESI) and quadrupole time-of-flight mass spectrometry (QToF/MS) [1]. UHPLC-DAD allows to separate and quantify simultaneously both major and minor phenolic compounds in the juices in a single run injection. Moreover, the identification of phenolic compounds was carried out by means of a Q-ToF/MS strategy using MSE scan mode [1]. Forty one phenolic compounds were quantified in the juices samples: 10 hydroxycinnamic acids, 18 flavan-3-ols and procyanidins, 7 dihydrochalcones and 6 flavonols. The apple juice polyphenolic profiles were analysed by pattern recognition techniques in order to (a) determine whether there are significant differences in their phenolic composition among harvests; (b) find out whether a specific genitor transmits a certain phenolic composition to its descendants and (c) learn whether a given descendant comes from a specific genitor.