Interaction between apple and bioagressors: Phenolic composition and the resistance to the rosy apple aphid

SASÍA-ARRIBA, A.; VICENTE-DIENTE, L.; RAMÍREZ-AMBROSI, M.; MIÑARRO, M.; BLAZQUEZ, M. D.; ALONSO SALCES, ROSA MARIA; MICHELETTI, D.; TROGGIO, M.; BERRUETA, L. A.; GALLO, B.; DAPENA, E.

Porto

Congreso; 10th World Congress on Polyphenols Applications; 2016

International Society of Antioxidants in Health & Nutrition

Polyphenols may play a role in plant response to biotic-stress and in resistance mechanisms. We aimed at testing the correlation between phenol concentration in apple and cultivar tolerance to rosy apple aphid (RAA), Dysaphis plantaginea, a key pest of apple on a full-sib family produced at SERIDA, derived of a crossing between the local cultivar ?Meana? and the resistant ?Florina?. The analysis of flavan-3 ols, hydroxycinnamic acids, dyhidrochalcones and flavonols by UHPLC-DAD was carried out on the two parents and 154 descendants. Their resistance to RAA was also determined after aphid inoculation in greenhouse. A relationship between the concentration of three caffeoylquinic acid isomers and 4-p-coumaroylquinic acid and the resistance to RAA was found by pattern recognition techniques. This progeny was also genotyped using the Illumina Infinium Apple_20k SNP array. A number of QTLs on linkage group 8 (LG8) associated with hydroxycinnamic acids were found. The RAA resistance locus was already known to be at the bottom of the LG8 and our results in ?Meana? x ?Florina? have confirmed this observation. Therefore, these results support an interaction between hydroxycinnamic acids and RAA resistance.