Comparison and Validation of two Sample Clean-up Methologies for the High Pressure Liquid Chromatography Determination of Polycyclic Aromatic Hydrocarbons in Edible Oils

BARRANCO, A.; ALONSO-SALCES, R. M.; BERRUETA, L. A.; GALLO, B.; VICENTE, F.; SAROBE, M.

Journal of separation science

WILEY-VCH, INC

Año: 2003 vol. 26 p. 1554 - 1562

1615-9314

An off-line high-performance normal-phase liquid chromatography procedure with a silica column followed by reversed-phase high-performance liquid chromatography (HPLC) with fluorescence detection for the determination of polycyclic aromatic hydrocarbons (PAHs) in edible oils is reported. The method was validated using certified reference materials and compared with a standardized method widely used in the food industry, consisting in low pressure column chromatography with alumina as stationary phase followed by reversed phase HPLC determination. The limits of detection were lower than 1 ng/g and good selectivity was achieved for both methods. There were no significant differences in accuracies and precisions obtained for each approach. The advantages and disadvantages of the two methods are discussed.