INVESTIGADORES
ALONSO SALCES Rosa Maria
artículos
Título:
Solid Phase Clean-up in the Liquid Chromatography for the Determination of Polycyclic Aromatic Hydrocarbons in Edible Oils
Autor/es:
BARRANCO, A.; ALONSO-SALCES, R. M.; BAKKALI, A.; BERRUETA, L. A.; GALLO, B.; VICENTE, F.; SAROBE, M.
Revista:
Journal of Chromatography - A
Editorial:
ELSEVIER
Referencias:
Año: 2003 vol. 988 p. 33 - 40
ISSN:
0021-9673
Resumen:
A solid-phase extraction (SPE) method for sample clean-up, followed by reversed-phase high-performance liquid chromatography (HPLC) with fluorescence detection is reported for the determination of polycyclic aromatic hydrocarbons (PAHs) in edible oils. The effects of experimental variables, such as washing and elution solvents, sample solvent and drying time have been studied using C18 cartridges. Recoveries and selectivity using other sorbent materials (C8, C2, CH, PH and NH2) were also examined, with C18 being the best one. The recoveries ranged between 50 and 103% depending on the molecular mass of the PAH. The limits of quantitation were lower than 1 ng/g for most PAHs and good precision was achieved. The method was validated using certified reference materials.
