Comparison of alcohol extraction of oil from oil-bearing products with different lipid compositions

YAO LINXING; LEE SHOW-LING; GERDE JOSE A.; WANG TONG

Long Beach, California

Conferencia; 103rd AOCS Annual Meeting & Expo; 2012

American Oil Chemists' Society

With an increasing interest in using microalgae as a source for biofuel, complete extraction of oil from algae is desirable. Various extraction methods were applied to extract oil from the cells, among which alcohols were attractive alternative solvents to hexane and other petroleum-based organic solvents. A procedure using isopropanol to extract algae oil was previously developed in our laboratory and it was found the sonication pretreatment and the algae lipid composition might be major factors that influenced the extraction efficiency. In this study, isopropanol extraction efficiency of oil from Scenedesmus spp. and Schizochytrium limacinum was compared with from Nannochloropsis and full-fat soy flour. Effects of sonication pretreatment and isopropanol concentration were examined. The neutral and polar lipid distribution in each alcohol extraction fraction was determined by HPLC analysis. Using sonicated algae cell, 87 and 95% aqueous IPA extracted greater than 90% of total oil, significantly higher than 70 and 50% IPA did. The yield of 70% IPA extraction varied with the type of starting materials.