Phage Biocontrol of Enteropathogenic and Shiga Toxin-Producing Escherichia coli during milk fermentation (seleccionado como Editor's Choice para encabezar el 'issue' del mes julio de 2013).

TOMAT, DAVID DAMIAN; MERCANTI, DIEGO JAVIER; BALAGUÉ, CLAUDIA; QUIBERONI, ANDREA DEL LUJÁN

LETTERS IN APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2013 vol. 57 p. 3 - 10

0266-8254

Two bacteriophages, isolated from faeces, were assayed as biocontrol agents of pathogenic Escherichia coli during milk fermentation. Phage DT1 was tested on the strain E. coli DH5alfa, one enteropathogenic E. coli (EPEC) strain and one Shiga toxigenic E. coli O157:H7 (STEC) strain. Phage DT6 was tested on two STEC strains (O157:H7 and non-O157). One additional assay was performed by using a cocktail of both phages against the O157:H7 STEC strain. Streptococcus thermophilus 10-C, the strain used as lactic starter, reached 10E9 CFU/ml after 4 h, while pH values fell to 4-5 after 8 h, regardless of the presence of E. coli strains and/or phages. In absence of phages, E. coli strains reached 4-6 log CFU/ml at 5-6h. Escherichia coli DH5alfa and O157:H7 STEC strains were rapidly and completely inactivated by phage DT1 and phage cocktail, respectively, while O157:H7 STEC was completely inactivated either by DT1 or by DT6, after 8 h. The EPEC strain was not detected at 1 h (