Jasmonic and salicylic acid induction during the interaction of RC 218 and on wheat spikes under greenhouse conditions Bacillus methylotrophicus Fusarium graminearum

JUAN M. PALAZZINI; N. YERKOVICH; FUMERO, M.V.; TORRES, ADRIANA; CHULZE, S.N.

FOZ DE IGUAZU

Congreso; 11th Congreso Internacional de Biología Molecular de Plantas (IPMB 2015); 2015

IPMB2015

Species within the Fusarium graminearumcomplex are the main pathogens causing Fusarium head blight (FHB), a devastating disease of wheat and barley worldwide. Bacillusmethylotrophicus RC218 has beenpreviously characterized as a biocontrol agent to control FHB and deoxynivalenol accumulation. The aim of the present study was: -to evaluate the effect of B. methylotrophicus RC 218 (Bmet), F. graminearum(Fgram) and their interaction, on the induction of the phytohormones salicylic (SA) and jasmonic acid (JA) at different period times after challenging wheat spikes. The plant hormones were measured by HPLCMS/MS. The production of JAwas induced after Fgram inoculation at 48 and 72 h,meanwhile JAwas reduced when compared to the co-inoculated treatment. No differences in JAor SA induction were observedBmet treatment and the control.Under the Fgram treatment,SAproduction was early induced at 12h and after this period,SAdecreased to the basal levels.Under co-inoculation treatment levels of SAand JAweresimilar to the control. In general, early induction of SAwas observed under the Fgram treatment, as it was shown for initial FHB basal resistance,meanwhile the late induction of JAreveals a defense state againstnecrotrophic pathogens such as F. graminearum. It is possible that the biocontrol agent B.methylotrophicusRC218 had reduced the pathogen activity and, therefore, the plant response.