Ecophysiology of Fusarium temperatum isolated from maize in Argentina

FUMERO, M.V.; SULYOK, MICHAEL; CHULZE, S.N.

FOOD ADDITIVES AND CONTAMINANTS

TAYLOR & FRANCIS LTD

Lugar: Londres; Año: 2015 vol. 33 p. 147 - 156

0265-203X

The effect of water activity (aw = 0.95, 0.98 and 0.995), temperature (15, 25 and 30°C), incubationtime (7, 14, 21 and 28 days), and their interactions on growth and moniliformin (MON), beauvericin(BEA), fusaproliferin (FUS) and fumonisin B1 (FB1) production by two strains of Fusariumtemperatum isolated from Argentinean maize were determined in vitro on sterile layers of maizegrains. The results showed that there was a wide range of conditions for growth and mycotoxinsproduction by F. temperatum. Both strains were found to grow faster with increasing aw and at30°C. In relation to mycotoxin production, the two strains produced more FUS than the othermycotoxins regardless of aw or temperature evaluated (maximum = 50 000 μg g−1). For FUS, MONand BEA, the maximum levels were observed at 0.98 aw and 30°C (50 000, 5000 and 2000 μg g−1respectively). The lowest levels for these three mycotoxins were detected at 15°C and 0.95 aw(1700 and 100 μg g−1 for FUS and MON respectively), and at 0.98 aw (400 μg g−1 for BEA). Themaximum levels of FB1 were produced at 15°C and 0.98 aw (1000 μg g−1). At all aw andtemperatures combinations evaluated there was an increase in toxin concentrations with timeincubation. The maximum levels were detected at 21 days. Statistical analyses of aw, temperature,incubation time, and the two- and three-way interactions between them showed significanteffects on mycotoxins production by F. temperatum. For its versatility on growth and mycotoxinproduction, F. temperatum represents a toxicological risk for maize in the field and also duringgrain storage.