Growth in milk with additives and determination of cell surface hydrophobicity of lactic acid bacteria of avian origin.

BLAJMAN J.E.; GAZIANO, C.P.; ASTESANA, D.M.; FUSARI, M.L.; BERISVIL, A.P.; ZIMMERMANN, J.A.; ZBRUN, M.V.; ROSMINI, M.R.; FRIZZO, L.S.

Zaballa

Congreso; XV Congreso y XXXIII Reunión Anual de la Sociedad de Biología de Rosario.; 2013

Sociedad de Biología de Rosario

In vitro and technological properties have to be taken into consideration in the selection process of probiotic microorganisms. This study compares the growth of three lactic acid bacteria strains of avian origin (Lactobacillus salivarius DSPV 001P, Lactobacillus salivarius DSPV 003P and Lactobacillus agilis DSPV 004P) in milk with additives and determine their cell surface hydrophobicity. Strains were inoculated in rehydrated skim milk powder + 5% w v yeast extract + 0.003% w v MnSO4 and incubated during 24 h at 37ºC. After decimal dilutions in Ringer ¼ solution, viable cell counts were determined by pour-plating method in MRS agar. The in vitro cell surface hydrophobicity was established using n-hexadecane. The percentage of cell surface hydrophobicity (%H) of the strain adhering to hexadecane was calculated using the equation: H%=[(A0 ? A)/A0] x (100), in which A0 and A represent the OD 600nm before and after extraction with n-hexadecane respectively. Strains can grow well in milk with additives (9.22 log10 CFU/ml for L. agilis DSPV 004P, 8.81 log10 CFU/ml for L. salivarius DSPV 001P and 8.53 log10 CFU/ml for L. salivarius DSPV 003P), and have moderate to high levels of hydrophobicity (72% for L. salivarius DSPV 003P, 69% for L. salivarius DSPV 001P and 63% for L. agilis DSPV 004P), associated with the ability to adhere to epithelial cells and mucosal surfaces.