Colonization of Lactobacillus salivarius DSPV 001P in the gastrointestinal tract of broilers.

BLAJMAN, J.E.; FUSARI, M.L.; ROMERO SCHARPEN, A.; ZIMMERMANN, J.A.; BLANCO, M.; MALMIERCA, M.; SEQUEIRA, J.G.; FRIZZO, L.S.

Rosario

Congreso; XVI Congress - XXXIV Annual Meeting Rosario Biology Society; 2014

Rosario Biology Society

Survival of probiotic bacteria through the gastrointestinal tract is crucial to exert a positive effect when administered in broilers. The aim of this study was to assess the colonization and persistence of lactic acid bacteria (LAB) of avian origin with probiotic properties in the gastrointestinal tract (GIT) of broilers for 44 days. Rifampicin resistant L. salivarius DSPV 001P was selected to monitor bacteria during the study. Ninety six 1 day old Cobb broilers were used in the trial. There were three replicates with 32 broilers per replicate. Experiment lasted for 44 days. The strain was administered to the diet at a dose of 1x1010 CFU/broiler during 9 days and 1x109 CFU/broiler during 7 days. On day 0, 48 h post administration and every seven days, six broilers (two per replicate) were slaughtered by cervical dislocation. To measure the GIT colonization, the number of CFU recovered from crop and caecum was determined. On day 0, there were no rifampicin resistant bacteria detected from broilers' crop and caecum. After two days of feeding, the strain was found at levels of approximately 3.73±1.88 log CFU/crop and 4.97±0.31 log CFU/caecum. Throughout the 16-days feeding period the level of L. salivarius DSPV 001P increased to 7.87±0.44 log CFU/crop and 7.41±0.35 log CFU/caecum. Total cell count on day 44 was 6.75±1.67 log CFU/crop and 6.22±2.26 log CFU/caecum. L. salivarius DSPV 001P is capable of persist in the GIT of broilers during a complete rearing. Probiotic strains persistence following cessation of administration is the ideal situation for producers, as the strains do not necessarily need to be used as ongoing in-feed additives.