INVESTIGADORES
MINNAARD Jessica
artículos
Título:
Disassembly of F-Actin Cytoskeleton after Interaction of Bacillus
Autor/es:
JESSICA MINNAARD, VANESSA LIEVIN-LE MOAL, MARIE-HELENE COCONNIER,ALAIN L. SERVIN, AND PABLO F. PEREZ
Revista:
INFECTION AND IMMUNITY
Editorial:
ASM
Referencias:
Año: 2004 vol. 72 p. 3106 - 3112
ISSN:
0019-9567
Resumen:
In the present study, the role of direct procaryote-eucaryote interactions in the virulence of Bacillus cereus
was investigated. As a model of human enterocytes, differentiated Caco-2 cells were used. Infection of fully
differentiated Caco-2 cells with B. cereus in the exponential phase of growth, in order to minimize the
concentration of spores or sporulating microorganisms, shows that a strain-dependent cytopathic effect
develops. Interestingly, addition of 3-h-old cultures of some strains resulted in complete detachment of the
cultured cells after a 3-h infection whereas no such effect was found after a 3-h infection with 16-h-old cultures.
Infection of enterocyte-like cells with B. cereus leads to disruption of the F-actin network and necrosis. Even
though the effect of secreted factors cannot be ruled out, direct eucaryote-procaryote interaction seems to be
necessary. In addition, we observed that some B. cereus strains were able to be internalized in Caco-2 cells. Our
findings add a new insight into the mechanisms of virulence of B. cereus in the context of intestinal infection