Prebiótico: su efecto sobre el crecimiento y capacidad de adhesión de cepas probióticas aviares

GUSILS, CARLOS; ROSS, GLORIA R.,; GONZÁLEZ SILVIA N.; APELLA, MARÍA C.

Buenos Aires

Congreso; XVII Congreso Latinoamericano de Microbiología, X Congreso Argentino de Microbiología; 2004

Asociación Argentina de Microbiología

Probiotic bacteria adhesion to the host´s gastrointestinal (GI) tract is a key prerequisite to achieve competitive exclusion of pathogenic bacteria and local and systemic modulation of the inmunological system. A prebiotic supplement affects the host beneficially by selectively stimulating growth and/or activity of one or a limited number of bacteria in the colon, especially Lactobacillus and Bifidobacterium. The aim of this study was to investigate in vitro adhesion of seven lactic acid bacteria (LAB) strains (isolated from poultry GI tract) with probiotic properties to chicken alimentary tract tissue sections (crop, ileum and cecum) as well as to ileal mucus and to establish the influence of raffinose or lactose addition on this process. The adhesion test was performed by incubating tissue samples (28 mm2) in bacterial suspensions (108-109 cells/mL PBS) at 42 °C for 60 min with or without prebiotic (1% w/v). Bacterial suspensions (100 ìL) were added to wells with immobilized mucus and incubated (42 °C, 1 h) for mucus adhesion assay. The number of bacterial cells attached was determined on LBS (lactobacilli) or KF agar (enterococci). All LAB tested were able to adhere to three epithelial surfaces and ileal mucus. L. fermentum CRL1015 showed the highest adhesion to epithelial fragments (86, 90 and 82% to crop, ileum and cecum, respectively) while L. reuteri CRL1454 was found to adhere better to mucus (25%). Prebiotic presence reduced tested LAB adhesion except for L. crispatus CRL1453 which increased its adhesion to the cecum ephitelium from 75 to 85%.