Antiparasitic activity of probiotic strains isolated from pigs

R. ROSS, ; E. ARGAÑARAZ MARTÍNEZ; C. GUSILS,; S. GONZÁLEZ.

San Miguel de Tucumán

Simposio; III Simposio Internacional de Bacterias Lácticas; 2009

Cerela-Conicet

ANTIPARASITIC ACTIVITY OF PROBIOTIC STRAINS ISOLATED FROM PIGS R. Ross1, E. Argañaraz Martínez3, C. Gusils2, S. González1, 3 1 Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán. Ayacucho 491 ? 4000 ? Tucumán, Argentina. Email:romiross23@yahoo.com.ar 2Estación Experimental Agroindustrial Obispo Colombres (EEAOC) - CONICET. Av. Williams Cross 3150 ? 4101 ? Las Talitas, Tucumán, Argentina. 1, 3 Centro de Referencia para Lactobacilos CERELA-CONICET. Chacabuco 145 ? 4000 ? Tucumán, Argentina. Parasite infections are widespread in swine, causing negative effects on production and high economic loss to producers. Gastrointestinal parasites cause anemia, decrease of growth performance parameters, depression of immunology system, and development of diseases that produce injures principally in lungs and liver in animals. An efficient control must prevent reinfestation and the producers usually use chemical parasiticides. Prolonged administration of these chemicals represents a potential risk for livestock (development of resistance), so it is an important alternative the design of specific probiotic foods to animals that could able eliminate or reduce the use of chemical parasiticides. The objective of the present work was to evaluate the antiparasitic probiotic effect, in vitro, of lactic acid bacteria strains isolated from pigs. Samples of pig faeces were collected from pens. To determinate the number of eggs presents per gram of faeces (e.p.g) it was used the McMaster counting technique that consist in separating eggs from faecal by a flotation fluid (saturated salt solution) in a counting chamber. To study the ovicide activity of a probiotic mix culture (Lactobacillus amylovorus and Enterococcus faecium), parasite eggs were sterilized with sodium hypochlorite solution, washed with sterilized distilled water and centrifugated at 2500 rpm four times to eliminate sodium hypochlorite residues. A defined parasite load was co-cultivated with a suspension of probiotic mix culture (108 CFU/ml) in LAPTg broth at 28°C during 24 hours. The same parasite load was incubated in LAPTg broth as control samples. After incubation, the development of parasite eggs was observed by microscopy. Nematode eggs, cestode eggs and coccidia oocysts were detected by a simple test tube flotation. There was a prevalence of nematode eggs and cestode eggs in comparison with coccidian oocysts. Probiotic culture caused the 100% removal of parasite eggs from the faecal samples; whereas only a 5% removal was observed in control samples. The results from the present study indicate that the probiotic mix culture has a high ovicide effect on nematode and cestode eggs, and coccidia oocysts. This inhibitory effect could probably due to the accumulation of antagonic metabolites produced by probiotic bacteria.