Estudio de integridad y biodisponibilidad de bacterias lácticas encapsuladas

ROSS, R; ; GUSILS, C;; GONZÁLEZ S

Tafi del Valle, Tucumán

Jornada; XXII Jornadas Científicas de Biología; 2005

Asociación de Biología de Tucumán

VIABILITY OF ENCAPSULATED LACTIC ACID BACTERIA Ross, G, Gusils, C y Gonzalez, S. Fac. Bqca, Qca. Y Fcia, UNT. Ayacucho 491-400-Tucumán, Argentina, Email; grossfbqf.unt.edu.ar Encapsulation is a process used to protect substances against oxidation, to improve their transport, etc. There is a wide kind of coating materials: hydrogenated oils, starches and gums like sodium alginate. The aim of this work was study the viability and survival in gastrointestinal conditions of probiotic lactic acid bacteria encapsulated in calcium alginate. The optimal encapsulation process was achieved using same relation (v/v) of non fat milk cell suspension (20%) and sodium alginate (1,8%). It was also added EDTA (1%) This mixture was aseptically dropped in calcium chloride solution (0.1M) bringing up capsules. The survival of encapsulated bacteria in simulated gastrointestinal conditions was studied Electron microscopy was used to demonstrated shape and structure of capsules and also presence of viable cells inside them. The results of this study indicated that calcium alginate encapsulation protects lactic acid bacteria of gastrointestinal conditions Our data demonstrated that calcium alginate capsules were able to keep viable cells inside them and therefore they could be used as a safe delivery vehicle for administering probiotic bacteria.