HYALURONAN MODULATION BY 4-METHYLUMBELLIFERONE (4MU) CONFERS CHEMO SENSIBILITY TO LUNG CANCER STEM CELLS

MARCO AURELIO DIAZ GUTIERREZ; ANA BEZAZIAN; PAULA ROSELLÓ; AMANDA ESCRIBANO; MARIEL FUSCO; JUAN BRAGA MENENDEZ; ESTEBAN FIORE; JUAN BAYO; MIGUEL RIZZO; MARIANA MALVICINI

Buenos Aires

Congreso; Reunion Anual de Biociencias 2020; 2020

SAI-SAFIS-SAIC

Taxane-platin chemotherapy is widely used for non-small cell lung cancer (NSCLC). However, the majority of patients will progress or relapse. In the tumor microenvironment (TME) cancer cells co-exist with cellular and non-cellular components that drive tumor processes such as chemotherapy resistance. Cancer stem cells (CSCs) are tumor initiating cells identified by CD133, CD44 and ALDH1 among others markers, which form residual niches involved in tumor recurrence. It has been partly described how the TME hyaluronan (HA) regulates CSCs function. We have demonstrated that 4-Methylumbelliferone (4Mu), a modulator of HA synthesis, reduces CSCs properties in hepatocarcinoma. Here, we observed that HA was present on mice Lewis Lung Carcinoma (LLC) tumors. We found HA+ LLC cells; thus cancer cells produced, at least in part, the HA observed in tumors. We observed about 6.58 ± 0.83 % of CD133+ CSCs on in vitro cultured LLC cells. Isolated CD133+ cells showed higher expression of HA and CD44 in comparison with non-CSCs population (p