INVESTIGADORES
MALVICINI Mariana
congresos y reuniones científicas
Título:
Low molecular weight hyaluronan-preconditioning of tumor-pulsed dendritic cells increases their migratory ability and induces immunity against murine colorectal carcinoma
Autor/es:
RIZZO, MANGLIO; GARCÍA, MARIANA; PICCIONI, FLAVIA; RODRIGUEZ, ANDRES; MALVICINI, MARIANA; KIPPES, NESTOR; ATORRASAGASTI, CATALINA; AQUINO, JORGE; ALANIZ, LAURA; MAZZOLINI, GUILLERMO
Lugar:
Buenos Aires
Reunión:
Congreso; First French-Argentine Immunology Congress, 2010; 2010
Institución organizadora:
Sociedad Argentina de Inmunología (SAI)
Resumen:
Colorectal carcinoma (CRC) is one of the leading causes of cancer
death worldwide and, unfortunately, there is no curative treatment
for patients not amenable to surgical resection. Dendritic
cells (DC) are the most potent specialized antigen-presenting
cells, ex-vivo generated, tumor-antigen loaded mature DC are
currently exploited to immunity against cancer. Hyaluronan (HA)
is a glycosaminoglycan found in almost all tissues, its functions
are size-dependent and the LMW HA form has been shown to
induce the expression of inflammatory genes in many types of
cells including DC and was shown to stimulate T cell responses
by activating DC. We have recently shown that systemic administration
of LMW HA, significantly reduces CRC growth in vivo,
response partially mediated by DC. To potentiate the ability of
DC loaded with tumor lysate (DC/LT) to induce immunity against
CRC in mice we aimed to study the effects of preconditioning
DC with LMW HA for therapeutic vaccination. The antitumor effect
of LMW HA ex vivo-stimulated DC was evaluated in an in
vivo BALB/c CT26 CRC model. When these LMW HA-treated CRC
tumor lysate-pulsed DC (DC/TL/LMW-HA) were administered to
tumor-bearing mice, a potent antitumor response was observed
when compared to DC pulsed with tumor lysate alone, significantly
reduced tumor growth (p