Interleukin-10 production and DNA polymorphisms in patients with Hemolytic Uremic Syndrome.

MONGELOS MA; PINEDA G; BURBALLA A; EXENI R; SANTIAGO A; FIORENTINO G; ABELLEYRO MM; ROSSETTI LC; DE BRASI CD; MARINA P; RAMOS MV

Mar del Plata

Congreso; Reunión Conjunta LXIII Sociedad Argentina de Investigación Clínica (SAIC) - LXVI Sociedad Argentina de Inmunología (SAI) ? Sociedad Argentina de Fisiología (SAFIS) 2018; 2018

Sociedad Argentina de Investigación Clínica (SAIC) - Sociedad Argentina de Inmunología (SAI) ? Sociedad Argentina de Fisiología (SAFIS)

Introduction: Development of Hemolytic Uremic Syndrome (HUS) is associated withShiga toxin and inflammatory response. However, anti-inflammatory mechanisms couldalso be triggered to limit inflammation in vivo, such as production of the regulatory cytokineIL-10. Individual differences in IL-10 secretion associated with IL10-promoter single-nucleotide polymorphisms (SNP), such as rs1800896 (Legacy notation: -1082A>G) inwhich A- and G-alleles show low and high IL10 expression, respectively.Objective: The aim of this work was to evaluate the production of IL-10 and the specificSNP -1082A>G in patients with HUS.Materials and Methods: Blood samples were collected from HUS patients during theacute period and from Healthy Children (HC). Plasma and mononuclear cells (MNC) wereisolated, and MNC were cultured with Medium or LPS (100ng/ml) for 20h. In parallel,absolute number of Monocytes (Mo) in MNC was calculated by flow cytometry. Proteinexpression of IL-10 was evaluated in plasma and MNC-supernatants by ELISA. DNA wasobtained from MNC and IL10 SNP -1082A>G analysis was performed by allele specific-PCR (as-PCR) using a PCR-control template on CTLA4.Results: Plasmatic IL-10 concentration was increased in HUS patients (Mean±SEM(pg/mL): HUS=270.9±117.8*, HC=82.1±7.1, n=15, *p<0.05). The absolute number of Mofrom HUS and HC was calculated (Mean±SEM= (0.12±0.03/0.07±0.02).10 6 Mo/mL).However, the levels of IL-10 secreted by MNC of both groups were similar after LPSstimulation (Mean±SEM (pg/mL: Basal/LPS (HUS=235±100/6470±1056*;HC=189±70/7630±1959*, n=5, *p<0.05 vs Basal). HUS(HC) DNA samples (n=8(8))showed genotypes: [A/A]=2(4), [G/G]=1(0), [A/G]=5(4),Conclusions: These preliminary results show that plasmatic IL-10 is increased in HUSduring acute period. Monocytes in MNC population suggests a tendency to be higher inHUS than HC according to previous reports, however preliminary results show similar IL-10 production. Although carrying the at-risk-allele [G] of IL10 SNP rs1800896 prefiguresabout twice risk of HUS vs HC, larger populations are needed to confirm this association.